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1

Low sensitivity of a urine LAM-ELISA in the diagnosis of pulmonary tuberculosis

Klaus Reither, Elmar Saathoff, Jutta Jung, Lilian T Minja, Inge Kroidl, Eiman Saad, Jim F Huggett, Elias N Ntinginya, Lucas Maganga, Leonard Maboko, Michael Hoelscher

Journal:

BMC Infectious Diseases

Year:

2009

Language:

english

File:

PDF, 565 KB

english, 2009

2

Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

Päivi Tissari, Alimuddin Zumla, Eveliina Tarkka, Sointu Mero, Laura Savolainen, Martti Vaara, Anne Aittakorpi, Sanna Laakso, Merja Lindfors, Heli Piiparinen, Minna Mäki, Caroline Carder, Jim Huggett,

Journal:

Year:

2010

Language:

english

File:

PDF, 114 KB

english, 2010

3

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses

Pavšič, Jernej, Devonshire, Alison S., Parkes, Helen, Schimmel, Heinz, Foy, Carole A., Karczmarczyk, Maria, Gutiérrez-Aguirre, Ion, Honeyborne, Isobella, Huggett, Jim F., McHugh, Timothy D., Milavec,

Journal:

Journal of Clinical Microbiology

Year:

2015

Language:

english

File:

PDF, 384 KB

english, 2015

4

How to make Mathematics Biology's next and better microscope

Huggett, Jim, O’Grady, Justin, Bustin, Stephen

Journal:

Biomolecular Detection and Quantification

Year:

2014

Language:

english

File:

PDF, 604 KB

english, 2014

5

qPCR primer design revisited

Bustin, Stephen, Huggett, Jim

Journal:

Biomolecular Detection and Quantification

Year:

2017

Language:

english

File:

PDF, 1.59 MB

english, 2017

6

Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing

Cleveland, Megan H., Anekella, Bharathi, Brewer, Michael, Chin, Pei-Ju, Couch, Heather, Delwart, Eric, Huggett, Jim, Jackson, Scott, Martin, Javier, Monpoeho, Serge, Morrison, Tom, Ng, Siemon H.S., Us

Journal:

Year:

2020

Language:

english

File:

PDF, 392 KB

english, 2020

7

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Huggett, Jim F, Benes, Vladimir, Bustin, Stephen A, Garson, Jeremy A, Harris, Karthyn, Kammel, Martin, Kubista, Mikael, McHugh, Timothy D, Moran-Gilad, Jacob, Nolan, Tania, Pfaffl, Michael W, Salit, M

Journal:

Clinical Chemistry

Year:

2020

File:

PDF, 534 KB

2020

8

Expression of apoptosis-related genes in an Ethiopian cohort study correlates with tuberculosis clinical status

Markos Abebe, T. Mark Doherty, Liya Wassie, Abraham Aseffa, Kidist Bobosha, Abebech Demissie, Martha Zewdie, Howard Engers, Peter Andersen, Louise Kim, Jim Huggett, Graham Rook, Lawrence K. Yamuah, Al

Journal:

European Journal of Immunology

Year:

2010

Language:

english

File:

PDF, 311 KB

english, 2010

9

Expression of IL-4 mRNA in peripheral blood mononuclear cells from normal donors in relation to expression of TLR2

Jung-Su Chang, Keertan Dheda, Jim F. Huggett, Louise U. Kim, Alimuddin Zumla, Graham A.W. Rook

Journal:

Immunology Letters

Year:

2006

Language:

english

File:

PDF, 130 KB

english, 2006

10

Tuberculosis: A Comprehensive Clinical Reference H. Simon Schaaf, Alimuddin I. Zumla, editors. WB Saunders Elsevier Ltd, London and New Delhi, 2009. 1014 pp., hardback, ISBN: 9781416039884 (£119.00). International edition for sale in developing countries: ISBN: 9788131221518 (£35.00)

Journal:

Transactions of the Royal Society of Tropical Medicine and Hygiene

Year:

2010

Language:

english

File:

PDF, 109 KB

english, 2010

11

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Stephen A Bustin, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning, Stefan Toegel

Journal:

BMC Molecular Biology

Year:

2010

Language:

english

File:

PDF, 427 KB

english, 2010

12

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis

Sanjay Lala, Keertan Dheda, Jung-Su Chang, Jim F Huggett, Louise U Kim, Margaret A Johnson, Graham AW Rook, Satish Keshav, Alimuddin Zumla

Journal:

BMC Infectious Diseases

Year:

2007

Language:

english

File:

PDF, 570 KB

english, 2007

13

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis

Anil Pooran, Helen Booth, Robert F Miller, Geoff Scott, Motasim Badri, Jim F Huggett, Graham Rook, Alimuddin Zumla, Keertan Dheda

Journal:

BMC Pulmonary Medicine

Year:

2010

Language:

english

File:

PDF, 1.41 MB

english, 2010

14

Standardisation and reporting for nucleic acid quantification

Jim Huggett, Stephen A. Bustin

Journal:

Accreditation and Quality Assurance

Year:

2011

Language:

english

File:

PDF, 403 KB

english, 2011

15

Problems of Developing Molecular Diagnostic Tests for Opportunistic Pathogens: The Example of Pneumocystis jirovecii

JIM F. HUGGETT, ROBERT F. MILLER, MARTIN S. TAYLOR, ANTHONY M. COSTELLO, ALIMUDDIN ZUMLA

Journal:

Journal of Eukaryotic Microbiology

Year:

2006

Language:

english

File:

PDF, 53 KB

english, 2006

16

The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4δ2, is not

Keertan Dheda, Jung-Su Chang, Jim F. Huggett, Louise U. Kim, Margaret A. Johnson, Alimuddin Zumla, Graham A.W. Rook

Journal:

Year:

2007

Language:

english

File:

PDF, 227 KB

english, 2007

17

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

Jim F Huggett, Tanya Novak, Jeremy A Garson, Clare Green, Stephen D Morris-Jones, Robert F Miller, Alimuddin Zumla

Journal:

BMC Research Notes

Year:

2008

Language:

english

File:

PDF, 371 KB

english, 2008

18

The open reading frame of the Na+-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain

Jim Huggett, Anne Vaughan-Thomas, Deborah Mason

Journal:

Year:

2000

Language:

english

File:

PDF, 302 KB

english, 2000

19

Crohn's disease and MAP

Jim Huggett, Keertan Dheda, Alimuddin Zumla, Graham Rook

Journal:

Year:

2004

Language:

english

File:

PDF, 60 KB

english, 2004

20

Future diagnosis of sepsis – Authors' reply

Vanya Gant, Martti Vaara, Minna Makki, Alimuddin Zumla, Jim Huggett

Journal:

Year:

2010

Language:

english

File:

PDF, 85 KB

english, 2010

21

Tuberculosis: amplification-based clinical diagnostic techniques

Jim F Huggett, Timothy D McHugh, Alimuddin Zumla

Journal:

The International Journal of Biochemistry & Cell Biology

Year:

2003

Language:

english

File:

PDF, 96 KB

english, 2003

22

Interferon gamma assays for tuberculosis

Keertan Dheda, Jung-Su Chang, Louise U Kim, Jim F Huggett, Margaret A Johnson, Alimuddin Zumla, Graham AW Rook

Journal:

The Lancet Infectious Diseases

Year:

2005

Language:

english

File:

PDF, 40 KB

english, 2005

23

Reflections on the white plague

Alimuddin Zumla, Peter Mwaba, Jim Huggett, Nathan Kapata, Duncan Chanda, John Grange

Journal:

The Lancet Infectious Diseases

Year:

2009

Language:

english

File:

PDF, 617 KB

english, 2009

24

Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods

Clare Green, Jim F Huggett, Elizabeth Talbot, Peter Mwaba, Klaus Reither, Alimuddin I Zumla

Journal:

The Lancet Infectious Diseases

Year:

2009

Language:

english

File:

PDF, 306 KB

english, 2009

25

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

Devonshire, Alison S., Sanders, Rebecca, Wilkes, Timothy M., Taylor, Martin S., Foy, Carole A., Huggett, Jim F.

Journal:

Year:

2013

Language:

english

File:

PDF, 685 KB

english, 2013

26

Evaluation of Digital PCR for Absolute DNA Quantification

Sanders, Rebecca, Huggett, Jim F., Bushell, Claire A., Cowen, Simon, Scott, Daniel J., Foy, Carole A.

Journal:

Analytical Chemistry

Year:

2011

Language:

english

File:

PDF, 2.99 MB

english, 2011

27

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac2SGL complexes fromMycobacterium tuberculosis-infected cells

Frank Camacho, Jim Huggett, Louise Kim, Juan F Infante, Marco Lepore…

Journal:

Year:

2013

Language:

english

File:

PDF, 277 KB

english, 2013

28

Considerations for the development and application of control materials to improve metagenomic microbial community profiling

Jim F. Huggett, Thomas Laver, Sasithon Tamisak…

Journal:

Accreditation and Quality Assurance

Year:

2013

Language:

english

File:

PDF, 222 KB

english, 2013

29

The need for transparency and good practices in the qPCR literature

Bustin, Stephen A, Benes, Vladimir, Garson, Jeremy, Hellemans, Jan, Huggett, Jim, Kubista, Mikael, Mueller, Reinhold, Nolan, Tania, Pfaffl, Michael W, Shipley, Gregory, Wittwer, Carl T, Schjerling, Pe

Journal:

Year:

2013

Language:

english

File:

PDF, 447 KB

english, 2013

30

Quality Assessment of Biobanked Nucleic Acid Extracts for Downstream Molecular Analysis

Wahlberg, Karin, Huggett, Jim, Sanders, Rebecca, Whale, Alexandra S., Bushell, Claire, Elaswarapu, Ramnath, Scott, Daniel J., Foy, Carole A.

Journal:

Biopreservation and Biobanking

Year:

2012

Language:

english

File:

PDF, 446 KB

english, 2012

31

Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

Nixon, Gavin, Garson, Jeremy A, Grant, Paul, Nastouli, Eleni, Foy, Carole A., Huggett, Jim F.

Journal:

Analytical Chemistry

Year:

2014

Language:

english

File:

PDF, 819 KB

english, 2014

32

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

Devonshire, Alison S., Whale, Alexandra S., Gutteridge, Alice, Jones, Gerwyn, Cowen, Simon, Foy, Carole A., Huggett, Jim F.

Journal:

Analytical and Bioanalytical Chemistry

Year:

2014

Language:

english

File:

PDF, 789 KB

english, 2014

33

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Sanders, Rebecca, Mason, Deborah J., Foy, Carole A., Huggett, Jim F.

Journal:

Analytical and Bioanalytical Chemistry

Year:

2014

Language:

english

File:

PDF, 1.22 MB

english, 2014

34

Lung Remodeling in Pulmonary Tuberculosis

Dheda, Keertan, Booth, Helen, Huggett, Jim F., Johnson, Margaret A., Zumla, Alimuddin, Rook, Graham A. W.

Journal:

The Journal of Infectious Diseases

Year:

2005

Language:

english

File:

PDF, 805 KB

english, 2005

35

Unreliable Real-Time PCR Analysis of Human Endogenous Retrovirus-W (HERV-W) RNA Expression and DNA Copy Number in Multiple Sclerosis

Garson, Jeremy A., Huggett, Jim F., Bustin, Stephen A., Pfaffl, Michael W., Benes, Vladimir, Vandesompele, Jo, Shipley, Gregory L.

Journal:

AIDS Research and Human Retroviruses

Year:

2009

Language:

english

File:

PDF, 102 KB

english, 2009

36

Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device

Kemp, Cordula, Birch, Christopher, Shaw, Kirsty J., Nixon, Gavin J., Docker, Peter T., Greenman, John, Huggett, Jim F., Haswell, Stephen J., Foy, Carole A., Dyer, Charlotte E.

Journal:

Analytical Methods

Year:

2012

Language:

english

File:

PDF, 219 KB

english, 2012

37

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods

Nixon, Gavin J., Svenstrup, Helle F., Donald, Carol E., Carder, Caroline, Stephenson, Judith M., Morris-Jones, Stephen, Huggett, Jim F., Foy, Carole A.

Journal:

Biomolecular Detection and Quantification

Year:

2014

Language:

english

File:

PDF, 678 KB

english, 2014

38

Highly Reproducible Absolute Quantification of Mycobacterium tuberculosis Complex by Digital PCR

Devonshire, Alison S., Honeyborne, Isobella, Gutteridge, Alice, Whale, Alexandra S., Nixon, Gavin, Wilson, Philip, Jones, Gerwyn, McHugh, Timothy D., Foy, Carole A., Huggett, Jim F.

Journal:

Analytical Chemistry

Year:

2015

Language:

english

File:

PDF, 478 KB

english, 2015

39

Making standards for quantitative real-time pneumococcal PCR

Morpeth, Susan C., Huggett, Jim F., Murdoch, David R., Scott, J. Anthony G.

Journal:

Biomolecular Detection and Quantification

Year:

2014

Language:

english

File:

PDF, 257 KB

english, 2014

40

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

Redshaw, Nicholas, Wilkes, Timothy, Whale, Alexandra, Cowen, Simon, Huggett, Jim, Foy, Carole

Journal:

Year:

2013

Language:

english

File:

PDF, 4.07 MB

english, 2013

41

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons

Olson, Nathan D., Lund, Steven P., Zook, Justin M., Rojas-Cornejo, Fabiola, Beck, Brian, Foy, Carole, Huggett, Jim, Whale, Alexandra S., Sui, Zhiwei, Baoutina, Anna, Dobeson, Michael, Partis, Lina, Mo

Journal:

Biomolecular Detection and Quantification

Year:

2015

Language:

english

File:

PDF, 875 KB

english, 2015

42

Assessing the Accuracy of Quantitative Molecular Microbial Profiling

O'Sullivan, Denise, Laver, Thomas, Temisak, Sasithon, Redshaw, Nicholas, Harris, Kathryn, Foy, Carole, Studholme, David, Huggett, Jim

Journal:

International Journal of Molecular Sciences

Year:

2014

Language:

english

File:

PDF, 662 KB

english, 2014

43

qPCR, dPCR, NGS – A journey

Huggett, Jim F., O’Grady, Justin, Bustin, Stephen

Journal:

Biomolecular Detection and Quantification

Year:

2015

Language:

english

File:

PDF, 1.84 MB

english, 2015

44

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

Redshaw, Nicholas, Huggett, Jim F, Taylor, Martin S, Foy, Carole A, Devonshire, Alison S

Journal:

Year:

2014

Language:

english

File:

PDF, 674 KB

english, 2014

45

In Vivo and In Vitro Studies of a Novel Cytokine, Interleukin 4δ2, in Pulmonary Tuberculosis

Dheda, Keertan, Chang, Jung-Su, Breen, Ronan A. M., Kim, Louise U., Haddock, Jamanda A., Huggett, Jim F., Johnson, Margaret A., Rook, Graham A. W., Zumla, Alimuddin

Journal:

American Journal of Respiratory and Critical Care Medicine

Year:

2005

Language:

english

File:

PDF, 179 KB

english, 2005

46

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients

Alikian, Mary, Ellery, Peter, Forbes, Martin, Gerrard, Gareth, Kasperaviciute, Dalia, Sosinsky, Alona, Mueller, Michael, Whale, Alexandra S., Milojkovic, Dragana, Apperley, Jane, Huggett, Jim F., Foro

Journal:

The Journal of Molecular Diagnostics

Year:

2016

Language:

english

File:

PDF, 1.01 MB

english, 2016

47

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL

Camacho, Frank, Sarmiento, María E., Reyes, Fatima, Kim, Louise, Huggett, Jim, Lepore, Marco, Otero, Oscar, Gilleron, Martine, Puzo, Germain, Norazmi, Mohd Nor, Rook, Graham, Mori, Lucia, De Libero, G

Journal:

International Journal of Mycobacteriology

Year:

2016

Language:

english

File:

PDF, 1.15 MB

english, 2016

48

Fundamentals of multiplexing with digital PCR

Whale, Alexandra S., Huggett, Jim F., Tzonev, Svilen

Journal:

Biomolecular Detection and Quantification

Year:

2016

Language:

english

File:

PDF, 1.46 MB

english, 2016

49

Expression of a novel cytokine, IL-4delta2, in HIV and HIV–tuberculosis co-infection

Dheda, Keertan, Chang, Jung-Su, Breen, Ronan AM, Haddock, Jamanda A, Lipman, Marc C, Kim, Louise U, Huggett, Jim F, Johnson, Margaret A, Rook, Graham AW, Zumla, Alimuddin

Journal:

Year:

2005

Language:

english

File:

PDF, 177 KB

english, 2005

50

Utility of the antigen-specific interferon-?? assay for the management of tuberculosis

Dheda, Keertan, Udwadia, Zarir F, Huggett, Jim F, Johnson, Margaret A, Rook, Graham A.W

Journal:

Current Opinion in Pulmonary Medicine

Year:

2005

Language:

english

File:

PDF, 223 KB

english, 2005