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1

Characterization of the Ornithine Hydroxylation Step in Albachelin Biosynthesis

Bufkin, Kendra, Sobrado, Pablo

Journal:

Year:

2017

Language:

english

File:

PDF, 1.70 MB

english, 2017

2

Basic region of residues 228–231 of protein kinase CK1α is involved in its interaction with axin: Binding to axin does not affect the kinase activity

Pablo Sobrado, Ana Jedlicki, Victor H. Bustos, Catherine C. Allende, Jorge E. Allende

Journal:

Journal of Cellular Biochemistry

Year:

2005

Language:

english

File:

PDF, 148 KB

english, 2005

3

Developmental expression of Xenopus myosin 1d and identification of a myo1d tail homology that overlaps TH1

Janine M. LeBlanc-Straceski, Anna Sokac, William Bement, Pablo Sobrado, Laura Lemoine

Journal:

Development, Growth & Differentiation

Year:

2009

Language:

english

File:

PDF, 2.33 MB

english, 2009

4

A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases

Jun Qi, Karina Kizjakina, Reeder Robinson, Karishma Tolani, Pablo Sobrado

Journal:

Analytical Biochemistry

Year:

2012

Language:

english

File:

PDF, 640 KB

english, 2012

5

Recombinant expression, purification, and characterization of ThmD, the oxidoreductase component of tetrahydrofuran monooxygenase

Michelle Oppenheimer, Brad S. Pierce, Joshua A. Crawford, Keith Ray, Richard F. Helm, Pablo Sobrado

Journal:

Archives of Biochemistry and Biophysics

Year:

2010

Language:

english

File:

PDF, 1.53 MB

english, 2010

6

Characterization of recombinant UDP-galactopyranose mutase from Aspergillus fumigatus

Michelle Oppenheimer, Myles B. Poulin, Todd L. Lowary, Richard F. Helm, Pablo Sobrado

Journal:

Archives of Biochemistry and Biophysics

Year:

2010

Language:

english

File:

PDF, 1.49 MB

english, 2010

7

Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase

Elvira Romero, Michael Fedkenheuer, Samuel W. Chocklett, Jun Qi, Michelle Oppenheimer, Pablo Sobrado

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2012

Language:

english

File:

PDF, 890 KB

english, 2012

8

Isolation and characterization of functional Leishmania major virulence factor UDP-galactopyranose mutase

Michelle Oppenheimer, Ana L. Valenciano, Pablo Sobrado

Journal:

Biochemical and Biophysical Research Communications

Year:

2011

Language:

english

File:

PDF, 303 KB

english, 2011

9

A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles

Pablo Sobrado, Michael A. Goren, Declan James, Carissa K. Amundson, Brian G. Fox

Journal:

Protein Expression and Purification

Year:

2008

Language:

english

File:

PDF, 1.23 MB

english, 2008

10

Analysis of the roles of amino acid residues in the flavoprotein tryptophan 2-monooxygenase modified by 2-oxo-3-pentynoate: characterization of His338, Cys339, and Cys511 mutant enzymes

Pablo Sobrado, Paul F Fitzpatrick

Journal:

Archives of Biochemistry and Biophysics

Year:

2002

Language:

english

File:

PDF, 251 KB

english, 2002

11

Analysis of the Role of the Active Site Residue Arg98 in the Flavoprotein Tryptophan 2-Monooxygenase, a Member of the l -Amino Oxidase Family †

Sobrado, Pablo, Fitzpatrick, Paul F.

Journal:

Year:

2003

Language:

english

File:

PDF, 149 KB

english, 2003

12

Aspergillus fumigatus SidA Is a Highly Specific Ornithine Hydroxylase with Bound Flavin Cofactor

Chocklett, Samuel W., Sobrado, Pablo

Journal:

Year:

2010

Language:

english

File:

PDF, 1.34 MB

english, 2010

13

Substrate Binding Modulates the Activity of Mycobacterium smegmatis G, a Flavin-Dependent Monooxygenase Involved in the Biosynthesis of Hydroxamate-Containing Siderophores

Robinson, Reeder, Sobrado, Pablo

Journal:

Year:

2011

Language:

english

File:

PDF, 1.52 MB

english, 2011

14

Structural Insight into the Mechanism of Oxygen Activation and Substrate Selectivity of Flavin-Dependent N-Hydroxylating Monooxygenases

Franceschini, Stefano, Fedkenheuer, Michael, Vogelaar, Nancy J., Robinson, Howard H., Sobrado, Pablo, Mattevi, Andrea

Journal:

Year:

2012

Language:

english

File:

PDF, 848 KB

english, 2012

15

Substrate-dependent dynamics of UDP-galactopyranose mutase: Implications for drug design

Boechi, Leonardo, de Oliveira, Cesar Augusto F., Da Fonseca, Isabel, Kizjakina, Karina, Sobrado, Pablo, Tanner, John J., McCammon, J. Andrew

Journal:

Protein Science

Year:

2013

Language:

english

File:

PDF, 2.18 MB

english, 2013

16

UDP-galactopyranose mutases from Leishmania species that cause visceral and cutaneous leishmaniasis

Fonseca, Isabel O., Kizjakina, Karina, Sobrado, Pablo

Journal:

Archives of Biochemistry and Biophysics

Year:

2013

Language:

english

File:

PDF, 2.75 MB

english, 2013

17

Arg279 is the key regulator of coenzyme selectivity in the flavin-dependent ornithine monooxygenase SidA

Robinson, Reeder, Franceschini, Stefano, Fedkenheuer, Michael, Rodriguez, Pedro J., Ellerbrock, Jacob, Romero, Elvira, Echandi, Maria Paulina, Martin del Campo, Julia S., Sobrado, Pablo

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2014

Language:

english

File:

PDF, 959 KB

english, 2014

18

Tryptophan-47 in the active site of Methylophaga sp. strain SK1 flavin-monooxygenase is important for hydride transfer

Han, Andre, Robinson, Reeder M., Badieyan, Somayesadat, Ellerbrock, Jacob, Sobrado, Pablo

Journal:

Archives of Biochemistry and Biophysics

Year:

2013

Language:

english

File:

PDF, 1.24 MB

english, 2013

19

Mechanistic studies on the flavin-dependent N6-lysine monooxygenase MbsG reveal an unusual control for catalysis

Robinson, Reeder M., Rodriguez, Pedro J., Sobrado, Pablo

Journal:

Archives of Biochemistry and Biophysics

Year:

2014

Language:

english

File:

PDF, 1.25 MB

english, 2014

20

C4a-Hydroperoxyflavin Formation in N -Hydroxylating Flavin Monooxygenases Is Mediated by the 2′-OH of the Nicotinamide Ribose of NADP +

Robinson, Reeder, Badieyan, Somayesadat, Sobrado, Pablo

Journal:

Year:

2013

Language:

english

File:

PDF, 621 KB

english, 2013

21

Structure, mechanism, and dynamics of UDP-galactopyranose mutase

Tanner, John J., Boechi, Leonardo, Andrew McCammon, J., Sobrado, Pablo

Journal:

Archives of Biochemistry and Biophysics

Year:

2014

Language:

english

File:

PDF, 5.09 MB

english, 2014

22

Probing the Relative Timing of Hydrogen Abstraction Steps in the Flavocytochrome b 2 Reaction with Primary and Solvent Deuterium Isotope Effects and Mutant Enzymes †

Sobrado, Pablo, Daubner, S. Colette, Fitzpatrick, Paul F.

Journal:

Year:

2001

Language:

english

File:

PDF, 87 KB

english, 2001

23

Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase

Dhatwalia, Richa, Singh, Harkewal, Solano, Luis M., Oppenheimer, Michelle, Robinson, Reeder M., Ellerbrock, Jacob F., Sobrado, Pablo, Tanner, John J.

Journal:

Journal of the American Chemical Society

Year:

2012

Language:

english

File:

PDF, 1.08 MB

english, 2012

24

Solvent and Primary Deuterium Isotope Effects Show That Lactate CH and OH Bond Cleavages Are Concerted in Y254F Flavocytochrome b 2 , Consistent with a Hydride Transfer Mechanism †

Sobrado, Pablo, Fitzpatrick, Paul F.

Journal:

Year:

2003

Language:

english

File:

PDF, 88 KB

english, 2003

25

Identification of Tyr413 as an Active Site Residue in the Flavoprotein Tryptophan 2-Monooxygenase and Analysis of Its Contribution to Catalysis †

Sobrado, Pablo, Fitzpatrick, Paul F.

Journal:

Year:

2003

Language:

english

File:

PDF, 164 KB

english, 2003

26

Crystal Structures of Trypanosoma cruzi UDP-Galactopyranose Mutase Implicate Flexibility of the Histidine Loop in Enzyme Activation

Dhatwalia, Richa, Singh, Harkewal, Oppenheimer, Michelle, Sobrado, Pablo, Tanner, John J.

Journal:

Year:

2012

Language:

english

File:

PDF, 2.93 MB

english, 2012

27

Identification of the Binding Region of the [2Fe-2S] Ferredoxin in Stearoyl-Acyl Carrier Protein Desaturase: Insight into the Catalytic Complex and Mechanism of Action †

Sobrado, Pablo, Lyle, Karen S., Kaul, Steven P., Turco, Michelle M., Arabshahi, Ida, Marwah, Ashok, Fox, Brian G.

Journal:

Year:

2006

Language:

english

File:

PDF, 552 KB

english, 2006

28

Mechanistic and Structural Studies of H373Q Flavocytochrome b 2 : Effects of Mutating the Active Site Base †,‡

Tsai, Chi-Lin, Gokulan, Kuppan, Sobrado, Pablo, Sacchettini, James C., Fitzpatrick, Paul F.

Journal:

Year:

2007

Language:

english

File:

PDF, 266 KB

english, 2007

29

gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O -glycan in Aspergillus nidulans and Aspergillus fumigatus

Komachi, Yuji, Hatakeyama, Shintaro, Motomatsu, Haruka, Futagami, Taiki, Kizjakina, Karina, Sobrado, Pablo, Ekino, Keisuke, Takegawa, Kaoru, Goto, Masatoshi, Nomura, Yoshiyuki, Oka, Takuji

Journal:

Molecular Microbiology

Year:

2013

Language:

english

File:

PDF, 4.58 MB

english, 2013

30

Contributions of Unique Active Site Residues of Eukaryotic UDP-Galactopyranose Mutases to Substrate Recognition and Active Site Dynamics

Da Fonseca, Isabel, Qureshi, Insaf A., Mehra-Chaudhary, Ritcha, Kizjakina, Karina, Tanner, John J., Sobrado, Pablo

Journal:

Year:

2014

Language:

english

File:

PDF, 4.10 MB

english, 2014

31

Mechanism of N -Hydroxylation Catalyzed by Flavin-Dependent Monooxygenases

Badieyan, Somayesadat, Bach, Robert D., Sobrado, Pablo

Journal:

Journal of Organic Chemistry

Year:

2015

Language:

english

File:

PDF, 4.60 MB

english, 2015

32

In Planta Variation of Volatile Biosynthesis: An Alternative Biosynthetic Route to the Formation of the Pathogen-Induced Volatile Homoterpene DMNT via Triterpene Degradation in Arabidopsis Roots

Sohrabi, Reza, Huh, Jung-Hyun, Badieyan, Somayesadat, Rakotondraibe, Liva Harinantenaina, Kliebenstein, Daniel J., Sobrado, Pablo, Tholl, Dorothea

Journal:

Year:

2015

Language:

english

File:

PDF, 1.49 MB

english, 2015

33

A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications

Guo, Sujuan, Liang, Yanping, Murphy, Susan F, Huang, Angela, Shen, Haihong, Kelly, Deborah F, Sobrado, Pablo, Sheng, Zhi

Journal:

Year:

2015

Language:

english

File:

PDF, 1.75 MB

english, 2015

34

An Unprecedented NADPH Domain Conformation in Lysine Monooxygenase NbtG Provides Insights into Uncoupling of Oxygen Consumption from Substrate Hydroxylation

Binda, Claudia, Robinson, Reeder M., Martin del Campo, Julia S., Keul, Nicholas D., Rodriguez, Pedro J., Robinson, Howard H., Mattevi, Andrea, Sobrado, Pablo

Journal:

Journal of Biological Chemistry

Year:

2015

Language:

english

File:

PDF, 2.50 MB

english, 2015

35

Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase

Qi, Jun, Oppenheimer, Michelle, Sobrado, Pablo

Journal:

Enzyme Research

Year:

2011

Language:

english

File:

PDF, 1.27 MB

english, 2011

36

Biosynthesis of Galactofuranose in Kinetoplastids: Novel Therapeutic Targets for Treating Leishmaniasis and Chagas' Disease

Oppenheimer, Michelle, Valenciano, Ana L., Sobrado, Pablo

Journal:

Enzyme Research

Year:

2011

Language:

english

File:

PDF, 517 KB

english, 2011

37

X-ray structure of a mammalian stearoyl-CoA desaturase

Bai, Yonghong, McCoy, Jason G., Levin, Elena J., Sobrado, Pablo, Rajashankar, Kanagalaghatta R., Fox, Brian G., Zhou, Ming

Journal:

Year:

2015

Language:

english

File:

PDF, 5.55 MB

english, 2015

38

Noncanonical Reactions of Flavoenzymes

Journal:

International Journal of Molecular Sciences

Year:

2012

Language:

english

File:

PDF, 6.67 MB

english, 2012

39

Contribution to catalysis of ornithine binding residues in ornithine N5-monooxygenase

Robinson, Reeder, Qureshi, Insaf A., Klancher, Catherine A., Rodriguez, Pedro J., Tanner, John J., Sobrado, Pablo

Journal:

Archives of Biochemistry and Biophysics

Year:

2015

Language:

english

File:

PDF, 722 KB

english, 2015

40

In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction

Mehra-Chaudhary, Ritcha, Dai, Yumin, Sobrado, Pablo, Tanner, John J.

Journal:

Year:

2016

Language:

english

File:

PDF, 867 KB

english, 2016

41

The Structure of the Antibiotic Deactivating, N-hydroxylating Rifampicin Monooxygenase

Liu, Li-Kai, Abdelwahab, Heba, Martin Del Campo, Julia S., Mehra-Chaudhary, Ritcha, Sobrado, Pablo, Tanner, John J.

Journal:

Journal of Biological Chemistry

Year:

2016

Language:

english

File:

PDF, 6.94 MB

english, 2016

42

Inhibition of the flavin-dependent monooxygenase Siderophore A (SidA) blocks siderophore biosynthesis and Aspergillus fumigatus growth

Martin Del Campo, Julia S, Vogelaar, Nancy, Tolani, Karishma, Kizjakina, Karina, Harich, Kim C., Sobrado, Pablo

Journal:

ACS Chemical Biology

Year:

2016

Language:

english

File:

PDF, 1.34 MB

english, 2016

43

Identification of structural determinants of NAD(P)H selectivity and lysine binding in lysine N6-monooxygenase

Abdelwahab, Heba, Robinson, Reeder, Rodriguez, Pedro, Adly, Camelia, El-Sohaimy, Sohby, Sobrado, Pablo

Journal:

Archives of Biochemistry and Biophysics

Year:

2016

Language:

english

File:

PDF, 1.73 MB

english, 2016

44

Multiple functionalities of reduced flavin in the non-redox reaction catalyzed by UDP-galactopyranose mutase

Sobrado, Pablo, Tanner, John J.

Journal:

Archives of Biochemistry and Biophysics

Year:

2017

Language:

english

File:

PDF, 2.79 MB

english, 2017

45

Sinorhizobium meliloti Chemotaxis to Multiple Amino Acids Is Mediated by the Chemoreceptor McpU

Webb, Benjamin A., Compton, K. Karl, del Campo, Julia S. Martin, Taylor, Doris, Sobrado, Pablo, Scharf, Birgit E.

Journal:

Molecular Plant-Microbe Interactions

Year:

2017

Language:

english

File:

PDF, 1.06 MB

english, 2017

46

Identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay

Martín del Campo, Julia S., Eckshtain-Levi, Meital, Sobrado, Pablo

Journal:

Biochemical and Biophysical Research Communications

Year:

2017

Language:

english

File:

PDF, 1.98 MB

english, 2017

47

Introduction to flavoproteins: Beyond the classical paradigms

Sobrado, Pablo, Gadda, Giovanni

Journal:

Archives of Biochemistry and Biophysics

Year:

2017

Language:

english

File:

PDF, 833 KB

english, 2017

48

Flavin-N5 Covalent Intermediate in the Non-redox Dehalogenation Reaction Catalyzed by an Atypical Flavoenzyme

Dai, Yumin, Kizjakina, Karina, Campbell, Ashley C, Korasick, David A, Tanner, John J, Sobrado, Pablo

Journal:

Year:

2017

Language:

english

File:

PDF, 3.76 MB

english, 2017

49

Flavin-N5 Covalent Intermediate in a Nonredox Dehalogenation Reaction Catalyzed by an Atypical Flavoenzyme

Dai, Yumin, Kizjakina, Karina, Campbell, Ashley C., Korasick, David A., Tanner, John J., Sobrado, Pablo

Journal:

Year:

2017

File:

PDF, 670 KB

2017

50

Author Correction: Identification of Aspergillus fumigatus UDP-Galactopyranose Mutase Inhibitors

Martin del Campo, Julia S., Eckshtain-Levi, Meital, Vogelaar, Nancy J., Sobrado, Pablo

Journal:

Scientific Reports

Year:

2018

Language:

english

File:

PDF, 664 KB

english, 2018