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1

A novel derivatization method with 5-bromonicotinic acid N-hydroxysuccinimide for determination of the amino acid sequences of peptides

Masaru Miyagi, Masayuki Nakao, Takashi Nakazawa, Ikunoshin Kato, Susumu Tsunasawa

Journal:

Rapid Communications in Mass Spectrometry

Year:

1998

Language:

english

File:

PDF, 180 KB

english, 1998

2

Methionine or not methionine at the beginning of a protein

Fred Sherman, John W. Stewart, Susumu Tsunasawa

Journal:

Year:

1985

Language:

english

File:

PDF, 731 KB

english, 1985

3

Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difluoride membrane by Western blotting

Dr. Hisashi Hirano, Setsuko Komatsu, Hideyuki Kajiwara, Yoshiaki Takagi, Susumu Tsunasawa

Journal:

ELECTROPHORESIS

Year:

1993

Language:

english

File:

PDF, 790 KB

english, 1993

4

A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry

Hiroki Kuyama, Kazuhiro Sonomura, Osamu Nishimura, Susumu Tsunasawa

Journal:

Analytical Biochemistry

Year:

2008

Language:

english

File:

PDF, 258 KB

english, 2008

5

Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment

Kazuhiro Sonomura, Hiroki Kuyama, Ei-ichi Matsuo, Susumu Tsunasawa, Shiroh Futaki, Osamu Nishimura

Journal:

Analytical Biochemistry

Year:

2011

Language:

english

File:

PDF, 650 KB

english, 2011

6

Reactivity of asparagine residue at the active site of the D105N mutant of fluoroacetate dehalogenase from Moraxella sp. B

Susumu Ichiyama, Tatsuo Kurihara, Yoshifumi Kogure, Susumu Tsunasawa, Haruhiko Kawasaki, Nobuyoshi Esaki

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2004

Language:

english

File:

PDF, 263 KB

english, 2004

7

A method for terminus proteomics: Selective isolation and labeling of N-terminal peptide from protein through transamination reaction

Kazuhiro Sonomura, Hiroki Kuyama, Ei-ichi Matsuo, Susumu Tsunasawa, Osamu Nishimura

Journal:

Bioorganic & Medicinal Chemistry Letters

Year:

2009

Language:

english

File:

PDF, 265 KB

english, 2009

8

Expression of human Cu, Zn-superoxide dismutase in an insect cell-free system and its structural analysis by MALDI-TOF MS

Toru Ezure, Takashi Suzuki, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Journal of Biotechnology

Year:

2009

Language:

english

File:

PDF, 547 KB

english, 2009

9

Preparation of ubiquitin-conjugated proteins using an insect cell-free protein synthesis system

Takashi Suzuki, Toru Ezure, Eiji Ando, Osamu Nishimura, Toshihiko Utsumi, Susumu Tsunasawa

Journal:

Journal of Biotechnology

Year:

2010

Language:

english

File:

PDF, 621 KB

english, 2010

10

Direct on-membrane peptide mass fingerprinting with MALDI–MS of tyrosine-phosphorylated proteins detected by immunostaining

Tsuyoshi Nakanishi, Eiji Ando, Masaru Furuta, Susumu Tsunasawa, Osamu Nishimura

Journal:

Journal of Chromatography B

Year:

2007

Language:

english

File:

PDF, 871 KB

english, 2007

11

Selective detection of 2-nitrobenzenesulfenyl-labeled peptides by matrix-assisted laser desorption/ionization-time of flight mass spectrometry using a novel matrix

Ei-ichi Matsuo, Chikako Toda, Makoto Watanabe, Noriyuki Ojima, Shunsuke Izumi, Koichi Tanaka, Susumu Tsunasawa, Osamu Nishimura

Journal:

Year:

2006

Language:

english

File:

PDF, 326 KB

english, 2006

12

N-Terminal protein modifications in an insect cell-free protein synthesis system and their identification by mass spectrometry

Takashi Suzuki, Masaaki Ito, Toru Ezure, Masamitsu Shikata, Eiji Ando, Toshihiko Utsumi, Susumu Tsunasawa, Osamu Nishimura

Journal:

Year:

2006

Language:

english

File:

PDF, 484 KB

english, 2006

13

Terminal proteomics: N- and C-terminal analyses for high-fidelity identification of proteins using MS

Takashi Nakazawa, Minoru Yamaguchi, Taka-aki Okamura, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2008

Language:

english

File:

PDF, 591 KB

english, 2008

14

Protein prenylation in an insect cell-free protein synthesis system and identification of products by mass spectrometry

Takashi Suzuki, Masaaki Ito, Toru Ezure, Masamitsu Shikata, Eiji Ando, Toshihiko Utsumi, Susumu Tsunasawa, Osamu Nishimura

Journal:

Year:

2007

Language:

english

File:

PDF, 405 KB

english, 2007

15

Expression of proteins containing disulfide bonds in an insect cell-free system and confirmation of their arrangements by MALDI-TOF MS

Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2007

Language:

english

File:

PDF, 586 KB

english, 2007

16

A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry

Hiroki Kuyama, Keisuke Shima, Kazuhiro Sonomura, Minoru Yamaguchi, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2008

Language:

english

File:

PDF, 640 KB

english, 2008

17

A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry

Hiroki Kuyama, Keisuke Shima, Kazuhiro Sonomura, Minoru Yamaguchi, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2008

File:

PDF, 168 KB

2008

18

A new approach for detecting C-terminal amidation of proteins and peptides by mass spectrometry in conjunction with chemical derivatization

Hiroki Kuyama, Chihiro Nakajima, Takashi Nakazawa, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2009

Language:

english

File:

PDF, 533 KB

english, 2009

19

Strategy for comprehensive identification of human N-myristoylated proteins using an insect cell-free protein synthesis system

Takashi Suzuki, Koko Moriya, Kei Nagatoshi, Yoshinobu Ota, Toru Ezure, Eiji Ando, Susumu Tsunasawa, Toshihiko Utsumi

Journal:

Year:

2010

Language:

english

File:

PDF, 532 KB

english, 2010

20

Improved 2-nitrobenzenesulfenyl method: optimization of the protocol and improved enrichment for labeled peptides

Ei-ichi Matsuo, Chikako Toda, Makoto Watanabe, Tetsuo Iida, Taro Masuda, Toshikazu Minohata, Eiji Ando, Susumu Tsunasawa, Osamu Nishimura

Journal:

Rapid Communications in Mass Spectrometry

Year:

2006

Language:

english

File:

PDF, 241 KB

english, 2006

21

Simultaneous detection of N-terminal fragment ions in a protein mixture using a ruthenium(II) complex

Akihiro Ito, Taka-aki Okamura, Hitoshi Yamamoto, Norikazu Ueyama, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Susumu Tsunasawa, Kojiro Ake, Ryoji Masui, Seiki Kuramitsu, Takashi Nakazawa, Shigemi Nori

Journal:

Rapid Communications in Mass Spectrometry

Year:

2007

Language:

english

File:

PDF, 345 KB

english, 2007

22

Specific isolation of N-terminal fragments from proteins and their high-fidelity de novo sequencing

Minoru Yamaguchi, Takashi Obama, Hiroki Kuyama, Daisuke Nakayama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Takashi Nakazawa, Shigemi Norioka, Osamu Nishimura, Susumu Tsunasawa

Journal:

Rapid Communications in Mass Spectrometry

Year:

2007

Language:

english

File:

PDF, 291 KB

english, 2007

23

An improved method for de novo sequencing of arginine-containing, Nα-tris(2,4,6-trimethoxyphenyl)phosphonium-acetylated peptides

Hiroki Kuyama, Kazuhiro Sonomura, Keisuke Shima, Osamu Nishimura, Susumu Tsunasawa

Journal:

Rapid Communications in Mass Spectrometry

Year:

2008

Language:

english

File:

PDF, 432 KB

english, 2008

24

Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids

Minoru Yamaguchi, Daisuke Nakayama, Keisuke Shima, Hiroki Kuyama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Takashi Nakazawa, Shigemi Norioka, Osamu Nishimura, Susumu Tsunasawa

Journal:

Rapid Communications in Mass Spectrometry

Year:

2008

Language:

english

File:

PDF, 313 KB

english, 2008

25

The specific isolation of C-terminal peptides of proteins through a transamination reaction and its advantage for introducing functional groups into the peptide

Kazuhiro Sonomura, Hiroki Kuyama, Ei-ichi Matsuo, Susumu Tsunasawa, Osamu Nishimura

Journal:

Rapid Communications in Mass Spectrometry

Year:

2009

Language:

english

File:

PDF, 300 KB

english, 2009

26

Erratum: The specific isolation of C-terminal peptides of proteins through the transamination reaction and its advantage for introducing functional groups into the peptide

Kazuhiro Sonomura, Hiroki Kuyama, Ei-ichi Matsuo, Susumu Tsunasawa, Osamu Nishimura

Journal:

Rapid Communications in Mass Spectrometry

Year:

2010

File:

PDF, 42 KB

2010

27

Nucleotide sequence of theliggene and primary structure of DNA ligase ofEscherichia coli

Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino, Susumu Tsunasawa, Fumio Sakiyama, Atsuo Nakata

Journal:

Molecular Genetics and Genomics

Year:

1986

Language:

english

File:

PDF, 634 KB

english, 1986

28

Determination of the covalent structure of aminoacylase from porcine kidney by ion-spray ionization mass spectrometry

Masaru Miyagi, Hiroshi Ohnogi, Masanori Mitta, Yoshihisa Umeda, Ikunoshin Kato, Fumio Sakiyama, Susumu Tsunasawa

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 255 KB

english, 1992

29

Cleavage of peptide bonds at CySO3H and CM-Cys with an endoprotease (blase) fromBacillus licheniformis

Shaoliang Li, Dorjbal Dorjsuren, Susumu Tsunasawa, Fumio Sakiyama

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 220 KB

english, 1992

30

Microsequence analysis of N-terminally blocked proteins

Susumu Tsunasawa, Hisashi Hirano

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 215 KB

english, 1992

31

Amino-terminal acetylation of altered form of yeast iso-1-cytochromescin normal andnat1−strains of yeast

Fred Sherman, Richard P. Moerschell, Susumu Tsunasawa, Rolf Sternglanz

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 379 KB

english, 1992

32

Sequence analysis of semidwarfism-related proteins in rice

Hisashi Hirano, Setsuko Komatsu, Atsushi Nakamura, Susumu Tsunasawa, Fumio Sakiyama

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 162 KB

english, 1992

33

Interisland Mutation of a Novel Phospholipase A2fromTrimeresurus flavoviridisVenom and Evolution of Crotalinae Group II Phospholipases A2

Takahito Chijiwa, Sachiko Hamai, Shoji Tsubouchi, Tomohisa Ogawa, Masanobu Deshimaru, Naoko Oda-Ueda, Shosaku Hattori, Hiroshi Kihara, Susumu Tsunasawa, Motonori Ohno

Journal:

Journal of Molecular Evolution

Year:

2003

Language:

english

File:

PDF, 358 KB

english, 2003

34

Efficient N-terminal peptide sequencing using bis(terpyridine)ruthenium(II) derivatives

Taka-aki Okamura, Norikazu Ueryama, Taku Iwamura, Mana Ikemori, Maki Kaneko, Ken Masui, Hitoshi Yamamoto, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Susumu Tsunasawa

Journal:

Journal of Inorganic Biochemistry

Year:

2003

Language:

english

File:

PDF, 110 KB

english, 2003

35

A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus

Takeshi Honda, Yuxin Ni, Schunkichi Hori, Hikaru Takakura, Susumu Tsunasawa, Fumino Sakiyama, Toshio Miwatani

Journal:

FEMS Microbiology Letters

Year:

1989

Language:

english

File:

PDF, 446 KB

english, 1989

36

Studies of disulfide bond location in ovine lutropin β subunit

Susumu Tsunasawa, Wan-Kyng Liu, Bruce D. Burleigh, Darrell N. Ward

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure

Year:

1977

Language:

english

File:

PDF, 959 KB

english, 1977

37

The N-terminal residue of bovine rhodopsin is acetylmethionine

Susumu Tsunasawa, Kozo Narita, Hitoschi Shichi

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure

Year:

1980

Language:

english

File:

PDF, 426 KB

english, 1980

38

The nucleotide sequence of human aminoacylase-1

Masanori Mitta, Ikunoshi Kato, Susumu Tsunasawa

Journal:

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

Year:

1993

Language:

english

File:

PDF, 214 KB

english, 1993

39

NMR evidence for the stepwise unfolding of the two domains of tryptophan synthase α-subunit

Hideo Iwahashi, Katsuhide Yutani, Kyoko Ogasahara, Susumu Tsunasawa, Yoshimasa Kyogoku, Yoshinobu Sugino

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1983

Language:

english

File:

PDF, 257 KB

english, 1983

40

The amino acid sequence of S-antigen: N-terminus and uveitogenic peptides

Susumu Tsunasawa, Hitoshi Shichi

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1989

Language:

english

File:

PDF, 340 KB

english, 1989

41

Purification and characterization of a novel solvent-tolerant lipase from Fusarium heterosporum

Yuji Shimada, Chigusa Koga, Akio Sugihara, Toshihiro Nagao, Nobuo Takada, Susumu Tsunasawa, Yoshio Tominaga

Journal:

Journal of Fermentation and Bioengineering

Year:

1993

Language:

english

File:

PDF, 465 KB

english, 1993

42

Lysine 190 is a covalent bilirubin-binding amino acid in human delta bilirubin

Yukihiko Adachi, Naomi Murata, Susumu Tsunasawa, Toshinori Kamisako, Toshio Yamamoto

Journal:

International Hepatology Communications

Year:

1996

Language:

english

File:

PDF, 388 KB

english, 1996

43

Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity

Ja Choon Koo, So Young Lee, Hyun Jin Chun, Yong Hwa Cheong, Jae Su Choi, Shun-ichiro Kawabata, Masaru Miyagi, Susumu Tsunasawa, Kwon Soo Ha, Dong Won Bae, Chang-deok Han, Bok Luel Lee, Moo Je Cho

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1998

Language:

english

File:

PDF, 663 KB

english, 1998

44

Mass spectrometric analysis of the reactions catalyzed by l-2-haloacid dehalogenase mutants and implications for the roles of the catalytic amino acid residues

Yong-Fu Li, Tatsuo Kurihara, Susumu Ichiyama, Masaru Miyagi, Susumu Tsunasawa, Nobuyoshi Esaki

Journal:

Journal of Molecular Catalysis B: Enzymatic

Year:

2003

Language:

english

File:

PDF, 139 KB

english, 2003

45

The inhibitory properties and primary structure of a novel serine proteinase inhibitor from the fruiting body of the basidiomycete, Lentinus edodes

Shoji Odani, Kei Tominaga, Satomi Kondou, Hiroshi Hori, Takehiko Koide, Saburo Hara, Mamoru Isemura, Susumu Tsunasawa

Journal:

Year:

1999

Language:

english

File:

PDF, 1013 KB

english, 1999

46

Characterization of novel cholesterol esterase from Trichoderma sp. AS59 with high ability to synthesize steryl esters

Atsushi Maeda, Takayuki Mizuno, Masanori Bunya, Shigeo Sugihara, Daisuke Nakayama, Susumu Tsunasawa, Yoshinori Hirota, Akio Sugihara

Journal:

Journal of Bioscience and Bioengineering

Year:

2008

Language:

english

File:

PDF, 373 KB

english, 2008

47

Studies on Soybean Trypsin Inhibitors : 2. Amino-Acid Sequence around the Reactive Site of Soybean Trypsin Inhibitor (Kunitz)

Takehiko Koide, Tokuji IKENAKA, Susumu Tsunasawa

Journal:

Year:

1973

Language:

english

File:

PDF, 758 KB

english, 1973

48

Complete amino acid sequence of mouse prolactin

Kaoru Kohmoto, Susumu Tsunasawa, Fumio Sakiyama

Journal:

Year:

1984

Language:

english

File:

PDF, 352 KB

english, 1984

49

Complete amino acid sequence of mouse prolactin

Kaoru KOHMOTO, Susumu TSUNASAWA, Fumio SAKIYAMA

Journal:

Year:

1984

Language:

english

File:

PDF, 1.04 MB

english, 1984

50

Effect of single amino acid substitutions on the protease susceptibility of tryptophan synthase a subunit

Kyoko OGASAHARA, Susumu TSUNASAWA, Yuji SODA, Katsuhide YUTANI, Yoshinobu SUGINO

Journal:

Year:

1985

Language:

english

File:

PDF, 478 KB

english, 1985

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