Volume 12; Issue 9

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Nature Protocols
Volume 12; Issue 9

Nature Protocols

Volume 12; Issue 9

1

The cubicon method for concentrating membrane proteins in the cubic mesophase

Ma, Pikyee, Weichert, Dietmar, Aleksandrov, Luba A, Jensen, Timothy J, Riordan, John R, Liu, Xiangyu, Kobilka, Brian K, Caffrey, Martin

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 2.51 MB

english, 2017

2

Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions

Chaturvedi, Sumit K, Ma, Jia, Zhao, Huaying, Schuck, Peter

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 2.82 MB

english, 2017

3

Mechanical isolation, and measurement of force and myoplasmic free [Ca2+] in fully intact single skeletal muscle fibers

Cheng, Arthur J, Westerblad, Håkan

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 2.59 MB

english, 2017

4

The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals

Cringoli, Giuseppe, Maurelli, Maria P, Levecke, Bruno, Bosco, Antonio, Vercruysse, Jozef, Utzinger, Jürg, Rinaldi, Laura

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 3.06 MB

english, 2017

5

Development of fertile mouse oocytes from mitotic germ cells in vitro

Morohaku, Kanako, Hirao, Yuji, Obata, Yayoi

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 6.36 MB

english, 2017

6

Reconstitution of mouse oogenesis in a dish from pluripotent stem cells

Hayashi, Katsuhiko, Hikabe, Orie, Obata, Yayoi, Hirao, Yuji

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 2.99 MB

english, 2017

7

Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication

Brödel, Andreas K, Jaramillo, Alfonso, Isalan, Mark

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 943 KB

english, 2017

8

Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells

Martell, Jeffrey D, Deerinck, Thomas J, Lam, Stephanie S, Ellisman, Mark H, Ting, Alice Y

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 5.65 MB

english, 2017

9

Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles

Kim, Ji-wook, Seo, Daeha, Lee, Jung-uk, Southard, Kaden M, Lim, Yongjun, Kim, Daehyun, Gartner, Zev J, Jun, Young-wook, Cheon, Jinwoo

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 2.90 MB

english, 2017

10

Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

Bao, Xiaoping, Lian, Xiaojun, Qian, Tongcheng, Bhute, Vijesh J, Han, Tianxiao, Palecek, Sean P

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 2.35 MB

english, 2017

11

Evaluation of telomere length in human cardiac tissues using cardiac quantitative FISH

Sharifi-Sanjani, Maryam, Meeker, Alan K, Mourkioti, Foteini

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 623 KB

english, 2017

12

Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis

Renfer, Eduard, Technau, Ulrich

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 1.59 MB

english, 2017

13

ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation

Bisceglie, Lavinia, Bartolomei, Giody, Hottiger, Michael O

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 733 KB

english, 2017

14

Detection of nucleic acid–protein interactions in plant leaves using fluorescence lifetime imaging microscopy

Camborde, Laurent, Jauneau, Alain, Brière, Christian, Deslandes, Laurent, Dumas, Bernard, Gaulin, Elodie

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 5.23 MB

english, 2017

15

Construction of an aerolysin nanopore in a lipid bilayer for single-oligonucleotide analysis

Cao, Chan, Liao, Dong-Fang, Yu, Jie, Tian, He, Long, Yi-Tao

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 3.45 MB

english, 2017

16

Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics

Baccouche, Alexandre, Okumura, Shu, Sieskind, Rémi, Henry, Elia, Aubert-Kato, Nathanaël, Bredeche, Nicolas, Bartolo, Jean-François, Taly, Valérie, Rondelez, Yannick, Fujii, Teruo, Genot, Anthony J

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 9.60 MB

english, 2017

17

Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion

Liu, Xiaoxia, Seven, Alpay Burak, Xu, Junjie, Esser, Victoria, Su, Lijing, Ma, Cong, Rizo, Josep

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 686 KB

english, 2017

18

Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation

Nolbrant, Sara, Heuer, Andreas, Parmar, Malin, Kirkeby, Agnete

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 11.04 MB

english, 2017

19

Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function

Kurachi, Makoto, Kurachi, Junko, Chen, Zeyu, Johnson, John, Khan, Omar, Bengsch, Bertram, Stelekati, Erietta, Attanasio, John, McLane, Laura M, Tomura, Michio, Ueha, Satoshi, Wherry, E John

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 1.83 MB

english, 2017

20

Production of tunable nanomaterials using hierarchically assembled bacteriophages

Lee, Ju Hun, Warner, Christopher M, Jin, Hyo-Eon, Barnes, Eftihia, Poda, Aimee R, Perkins, Edward J, Lee, Seung-Wuk

Journal:

Nature Protocols

Year:

2017

Language:

english

File:

PDF, 4.50 MB

english, 2017