Volume 2; Issue 12

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Nature Protocols
Volume 2; Issue 12

Nature Protocols

Volume 2; Issue 12

1

Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange

Ferlini, Cristiano, Scambia, Giovanni

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 187 KB

english, 2007

2

Pd-catalyzed Suzuki–Miyaura reactions of aryl halides using bulky biarylmonophosphine ligands

Altman, Ryan A, Buchwald, Stephen L

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 337 KB

english, 2007

3

Imaging activation of adult-generated granule cells in spatial memory

Kee, Nohjin, Teixeira, Cátia M, Wang, Afra H, Frankland, Paul W

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 345 KB

english, 2007

4

Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila

Ninov, Nikolay, Martín-Blanco, Enrique

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 423 KB

english, 2007

5

Induction of pluripotent stem cells from fibroblast cultures

Takahashi, Kazutoshi, Okita, Keisuke, Nakagawa, Masato, Yamanaka, Shinya

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 433 KB

english, 2007

6

Measurement of cell proliferation by heavy water labeling

Busch, Robert, Neese, Richard A, Awada, Mohamad, Hayes, Gregory M, Hellerstein, Marc K

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 325 KB

english, 2007

7

Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio–mass spectrometric analysis

Voogt, Jason N, Awada, Mohamad, Murphy, Elizabeth J, Hayes, Gregory M, Busch, Robert, Hellerstein, Marc K

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 166 KB

english, 2007

8

Site-selective glycosylation of proteins: creating synthetic glycoproteins

van Kasteren, Sander I, Kramer, Holger B, Gamblin, David P, Davis, Benjamin G

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 417 KB

english, 2007

9

Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization

Sainlos, Matthieu, Imperiali, Barbara

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 395 KB

english, 2007

10

Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach

Sainlos, Matthieu, Imperiali, Barbara

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 252 KB

english, 2007

11

Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN

Sainlos, Matthieu, Imperiali, Barbara

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 168 KB

english, 2007

12

Efficient transfection of DNA or shRNA vectors into neurons using magnetofection

Buerli, Thomas, Pellegrino, Christophe, Baer, Kristin, Lardi-Studler, Barbara, Chudotvorova, Ilona, Fritschy, Jean-Marc, Medina, Igor, Fuhrer, Christian

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 852 KB

english, 2007

13

Construction and calibration of an optical trap on a fluorescence optical microscope

Lee, Woei Ming, Reece, Peter J, Marchington, Robert F, Metzger, Nikolaus K, Dholakia, Kishan

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 558 KB

english, 2007

14

The Bead blot: a method for selecting small molecule ligands for protein capture and purification

Lathrop, Julia Tait, Hammond, David

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 309 KB

english, 2007

15

Synthesis of the 3′-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3′-S-phosphorothiolate linkage

Gaynor, James W, Bentley, Joanne, Cosstick, Richard

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 292 KB

english, 2007

16

Preparation of macromolecular complexes for cryo-electron microscopy

Grassucci, Robert A, Taylor, Derek J, Frank, Joachim

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 565 KB

english, 2007

17

Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences

Coin, Irene, Beyermann, Michael, Bienert, Michael

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 286 KB

english, 2007

18

Subtractive single-chain antibody (scFv) phage-display: tailoring phage-display for high specificity against function-specific conformations of cell membrane molecules

Eisenhardt, Steffen U, Schwarz, Meike, Bassler, Nicole, Peter, Karlheinz

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 380 KB

english, 2007

19

Profiling of microRNA expression by mRAP

Takada, Shuji, Mano, Hiroyuki

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 364 KB

english, 2007

20

Silencing of mammalian genes by tetracycline-inducible shRNA expression

Kappel, Sven, Matthess, Yves, Kaufmann, Manfred, Strebhardt, Klaus

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 382 KB

english, 2007

21

Detecting protein–protein interactions by far western blotting

Wu, Yuliang, Li, Qiang, Chen, Xing-Zhen

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 189 KB

english, 2007

22

Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system

Belur, Lalitha R, Podetz-Pedersen, Kelly, Frandsen, Joel, McIvor, R Scott

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 243 KB

english, 2007

23

Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection

Bell, Jason B, Podetz-Pedersen, Kelly M, Aronovich, Elena L, Belur, Lalitha R, McIvor, R Scott, Hackett, Perry B

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 761 KB

english, 2007

24

Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses

Füger, Petra, Behrends, Laila B, Mertel, Sara, Sigrist, Stephan J, Rasse, Tobias M

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 552 KB

english, 2007

25

Circular dichroism to determine binding mode and affinity of ligand–DNA interactions

Garbett, Nichola C, Ragazzon, Patricia A, Chaires, Jonathan B

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 274 KB

english, 2007

26

Single-cell isolation from cell suspensions and whole genome amplification from single cells to provide templates for CGH analysis

Geigl, Jochen B, Speicher, Michael R

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 344 KB

english, 2007

27

Synthesis of cationic single-isomer cyclodextrins for the chiral separation of amino acids and anionic pharmaceuticals

Tang, Weihua, Ng, Siu-Choon

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 169 KB

english, 2007

28

Purification and characterization of transcribed RNAs using gel filtration chromatography

McKenna, Sean A, Kim, Insil, Puglisi, Elisabetta Viani, Lindhout, Darrin A, Aitken, Colin Echeverría, Marshall, R Andrew, Puglisi, Joseph D

Journal:

Nature Protocols

Year:

2007

Language:

english

File:

PDF, 244 KB

english, 2007

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