Volume 34; Issue 1

Protein Expression and Purification

Volume 34; Issue 1

1

Deletion of the GPI pre-anchor sequence in human p97—a general approach for generating the soluble form of GPI-linked proteins

Joseph Yang, Jacqueline Tiong, Malcolm Kennard, Wilfred A Jefferies

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 1.06 MB

english, 2004

2

Selection of an Escherichia coli host that expresses mutant forms of Mycobacterium tuberculosis 2-trans enoyl-ACP(CoA) reductase and 3-ketoacyl-ACP(CoA) reductase enzymes

Simone S Poletto, Isabel O da Fonseca, Luiz P.S de Carvalho, Luiz A Basso, Diógenes S Santos

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 308 KB

english, 2004

3

Development of a large-scale HPLC-based purification for the urease from Staphylococcus leei and determination of subunit structure

Ming Jin, Wildys Rosario, Elsie Watler, David H Calhoun

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 236 KB

english, 2004

4

Cloning, high-level expression, single-step purification, and binding activity of His6-tagged recombinant type B botulinum neurotoxin heavy chain transmembrane and binding domain

Yu Zhou, Bal Ram Singh

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 247 KB

english, 2004

5

Cloning, high level expression, purification, and crystallization of the full length Clostridium botulinum neurotoxin type E light chain

Rakhi Agarwal, Subramaniam Eswaramoorthy, Desigan Kumaran, John J Dunn, Subramanyam Swaminathan

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 264 KB

english, 2004

6

High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase

Esther W Hou, Rajendra Prasad, William A Beard, Samuel H Wilson

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 380 KB

english, 2004

7

Expression, purification, crystallization, and preliminary X-ray analysis of the N-terminal domain of Escherichia coli adenylyl transferase

Yibin Xu, Daying Wen, Paula Clancy, Paul D. Carr, David L. Ollis, Subhash G. Vasudevan

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 202 KB

english, 2004

8

First structural investigation of the restriction ribonuclease RegB: NMR spectroscopic conditions, 13C/15N double-isotopic labelling and two-dimensional heteronuclear spectra

Fakhri Saı̈da, Benoı̂t Odaert, Marc Uzan, François Bontems

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 1.05 MB

english, 2004

9

Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells

Jason R Waggoner, Jamie Huffman, Brian N Griffith, Larry R Jones, James E Mahaney

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 927 KB

english, 2004

10

Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization

Zhiyong Zhou, Paul Schnake, Lihua Xiao, Altaf A. Lal

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 212 KB

english, 2004

11

Expression, purification, and characterization of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus

Linley R Schofield, Mark L Patchett, Emily J Parker

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 289 KB

english, 2004

12

Expression of the GM2-activator protein in the methylotrophic yeast Pichia pastoris, purification, isotopic labeling, and biophysical characterization

Michaela Wendeler, Joerg Hoernschemeyer, Michael John, Norbert Werth, Maike Schoeniger, Thorsten Lemm, Rudolf Hartmann, Horst Kessler, Konrad Sandhoff

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 292 KB

english, 2004

13

Expression, purification, and aggregation studies of His-tagged thermoalkalophilic lipase from Bacillus thermocatenulatus

Nils Helge Schlieben, Karsten Niefind, Dietmar Schomburg

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 226 KB

english, 2004

14

Overexpression and rapid purification of Escherichia coli formamidopyrimidine–DNA glycosylase

Prasad Reddy, Pawel Jaruga, Tim O’Connor, Henry Rodriguez, Miral Dizdaroglu

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 216 KB

english, 2004

15

The diagnostic performance of recombinant Trypanosoma cruzi ribosomal P2β protein is influenced by its expression system

Iván S Marcipar, Marı́a Laura Olivares, Lucı́a Robles, Andrés Dekanty, Alberto Marcipar, Ariel M Silber

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 175 KB

english, 2004

16

Heterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems

T Pechan, P.W.K Ma, D.S Luthe

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 173 KB

english, 2004

17

Non-fusion expression in Escherichia coli, purification, and characterization of a novel Ca2+- and phospholipid-binding protein annexin B1

Yi Zhang, Ying-jun Guo, Shu-han Sun, Hong-li Yan, Yan He

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 207 KB

english, 2004

18

Structural genomics for Caenorhabditis elegans: high throughput protein expression analysis

James B Finley, Shi-Hong Qiu, Chi-Hao Luan, Ming Luo

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 240 KB

english, 2004

19

Editorial Board

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 148 KB

english, 2004

20

Papers to Appear in Forthcoming Issues

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 77 KB

english, 2004