Volume 77; Issue 1

Protein Expression and Purification

Volume 77; Issue 1

1

The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: Insights into their use as tools for the removal of affinity tags

Brian P. Austin, József Tözsér, Péter Bagossi, Joseph E. Tropea, David S. Waugh

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 710 KB

english, 2011

2

Screening of genetic parameters for soluble protein expression in Escherichia coli

Erik Vernet, Alexander Kotzsch, Bjørn Voldborg, Michael Sundström

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 655 KB

english, 2011

3

Expression and characterization of recombinant Locusta migratoria manilensis acetylcholinesterase 1 in Pichia pastoris

Xiaoxia Zhou, Yuxian Xia

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 366 KB

english, 2011

4

Expression, purification, and refolding of biologically active Acinetobacter baumannii OmpA from Escherichia coli inclusion bodies

Michael J. McConnell, Jerónimo Pachón

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 514 KB

english, 2011

5

Model based optimization of the fed-batch production of a highly active transglutaminase variant in Escherichia coli

Christian Sommer, Norbert Volk, Markus Pietzsch

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 666 KB

english, 2011

6

FhuA deletion variant Δ1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer

Tamara Dworeck, Anne-Kathrin Petri, Noor Muhammad, Marco Fioroni, Ulrich Schwaneberg

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 347 KB

english, 2011

7

Refolding single-chain antibody (scFv) using lauroyl-l-glutamate as a solubilization detergent and arginine as a refolding additive

Motonori Kudou, Daisuke Ejima, Haruna Sato, Ryosuke Yumioka, Tsutomu Arakawa, Kouhei Tsumoto

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 679 KB

english, 2011

8

High-level soluble expression of recombinant human manganese superoxide dismutase in Escherichia coli, and its effects on proliferation of the leukemia cell

Wang Feng, Shao Mei, Yang Wenjie, Huang Luyuan

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 874 KB

english, 2011

9

High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins

Kimihiko Mizutani, Soshi Yoshioka, Yukiko Mizutani, So Iwata, Bunzo Mikami

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 694 KB

english, 2011

10

Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems

Mohammad Dadashipour, Yasuhisa Fukuta, Yasuhisa Asano

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 288 KB

english, 2011

11

The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography

Elizabeth S. Stalder, Lauren H. Nagy, Pilar Batalla, Terrance M. Arthur, Nancy E. Thompson, Richard R. Burgess

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 758 KB

english, 2011

12

Expression and purification of human FROUNT, a common cytosolic regulator of CCR2 and CCR5

Kaori Esaki, Yuya Terashima, Etsuko Toda, Sosuke Yoshinaga, Norie Araki, Kouji Matsushima, Hiroaki Terasawa

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 686 KB

english, 2011

13

Expression of the HSV-1 capsid protein VP19C in Escherichia coli: A single amino acid change overcomes an expression block of the full-length polypeptide

Brandon W. Henson, Nicole Johnson, Alakesh Bera, Mercy E. Okoye, Keshal Viren Desai, Prashant J. Desai

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 580 KB

english, 2011

14

Heterologous expression of Translocated promoter region protein, Tpr, identified as a transcription factor from Rattus norvegicus

Shivani Agarwal, Sunita Kumari Yadav, Aparna Dixit

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 686 KB

english, 2011

15

Expression of Apostichopus japonicus lysozyme in the methylotrophic yeast Pichia pastoris

Tingting Wang, Yongping Xu, Wenjia Liu, Yongxin Sun, Liji Jin

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 580 KB

english, 2011

16

New ligation-independent cloning vectors compatible with a high-throughput platform for parallel construct expression evaluation using baculovirus-infected insect cells

William Clay Brown, James DelProposto, J. Ronald Rubin, Kelly Lamiman, Jacob Carless, Janet L. Smith

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 1.18 MB

english, 2011

17

A chimera of green fluorescent protein with single chain variable fragment antibody against ginsenosides for fluorescence-linked immunosorbent assay

Seiichi Sakamoto, Yusuke Tanizaki, Benyakan Pongkitwitoon, Hiroyuki Tanaka, Satoshi Morimoto

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 520 KB

english, 2011

18

Identification of TMEM45B as a protein clearly showing thermal aggregation in SDS–PAGE gels and dissection of its amino acid sequence responsible for this aggregation

Naoto Okada, Takenori Yamamoto, Masahiro Watanabe, Yuuya Yoshimura, Eriko Obana, Naoshi Yamazaki, Kazuyoshi Kawazoe, Yasuo Shinohara, Kazuo Minakuchi

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 424 KB

english, 2011

19

IFC/ Editorial Board

Journal:

Protein Expression and Purification

Year:

2011

Language:

english

File:

PDF, 47 KB

english, 2011

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